What is the role of guanidine thiocyanate in New Coronavirus nucleic acid detection?

Recently, published in the "international consensus on the clinical application of New Coronavirus nucleic acid and antibody detection" published in the International Journal of laboratory medicine, it is mentioned that in the process of New Coronavirus acquisition, a sample tube containing virus inactivators such as guanidinium thiocyanate is recommended to inactivate the virus and increase the detection rate. What is the role of guanidine thiocyanate in COVID-19 nucleic acid detection, and how to inactivate the virus and increase the detection rate?

New Coronavirus is a kind of RNA virus. The nucleic acid of various RNA viruses is difficult to maintain stable biological molecules due to self degradation and enzyme mediated degradation. Guanidine thiocyanate is a kind of powerful protein denaturant, and has the characteristics of no RNase and DNase activity. Adding guanidine thiocyanate into the sampling tube can destroy the molecular structure of RNase, inactivate the nuclease that may exist in the sample, and play the role of stable preservation of virus samples.

COVID-19 nucleic acid detection process includes sampling, preservation, sample delivery, virus inactivation, cracking nucleic acid extraction and detection. The guanidine thiocyanate also plays a crucial role in nucleic acid extraction. As the main component of nucleic acid lysate, guanidine thiocyanate can quickly break the virus, quickly separate nuclear protein from nucleic acid, inhibit the released nuclease activity, ensure the integrity of the primary structure of nucleic acid and improve the efficiency of nucleic acid extraction.

Therefore, guanidine thiocyanate is of great value in the detection of COVID-19 nucleic acid.

Novel coronavirus pneumonia has been issued in recent years to respond to the joint guidance and control mechanism of the State Council. The efforts are being made to improve the nucleic acid detection capacity and expand the scope of nucleic acid detection, and to effectively detect the "focus of the disease" and other groups of people to "check and check".

With the development of large-scale nucleic acid detection, the demand for nucleic acid detection reagents and raw materials is also growing. It is understood that guanidine thiocyanate, guanidine hydrochloride and other nucleic acid detection reagent raw materials have become in short supply. As a national high-tech enterprise with 17 years of technical endorsement and product precipitation, Yake technology can stably provide guanidine thiocyanate products with various packaging specifications and excellent quality, help the development of nucleic acid detection reagent products, promote large-scale nucleic acid detection, and welcome customers from all walks of life to consult.