Guanidine thiocyanate 593-84-0 facilitates the extraction of nucleotides and peptides from animal viscera

Guanidine thiocyanate | 593-84-0 | is a strong protein denaturant. It is a white crystalline powder, irritating and sensitive to light. It is soluble in ethanol and water, with a melting point of 118 ℃. Guanidine thiocyanate is mainly used for denaturation, lysis of cells and extraction of RNA and DNA. It is a common chemical component for nucleic acid extraction.

Patent cn111500664a discloses a method for extracting nucleotides and polypeptides from animal viscera using guanidine thiocyanate. Nucleotides are compounds composed of purine base or pyrimidine base, ribose or deoxyribose and phosphoric acid, which are widely used in medicine, mother and child products, condiments and food additives. Polypeptide is α- Compounds formed by amino acids linked together by peptide bonds are intermediate products of protein hydrolysis, involving various fields of human hormones, nerves, cell growth and reproduction. Their importance lies in regulating the physiological functions of various systems and cells in the body, activating relevant enzymes in the body, promoting the permeability of intermediate metabolic membrane, or controlling DNA transcription or affecting specific protein synthesis, Eventually produce specific physiological effects.

Animal viscera are rich in nucleotides and peptides, but at present, the extraction of nucleotides and peptides is mostly carried out separately, resulting in waste of resources and complex processes. Guanidine thiocyanate is helpful to extract nucleotides and peptides from animal viscera. The specific methods are as follows:

First, wash the viscera of fresh animals, drain them, chop them up, then mash them, add deionized water to obtain the mixed solution, then add guanidine thiocyanate solution to the mixed solution, vibrate and mix well, and then stand. Then, a carrier containing silica is added to it, because guanidine thiocyanate will cleave the cells, and the nucleotides released from the cells will be combined with the silica carrier. Take out the carrier containing silica, absorb and separate it with eluent to obtain purified nucleotides.

Then, NaCl solution is added to the residual solution from which the silica carrier has been taken out, the mixed solution is extracted, then composite protease is added, then microwave enzymatic hydrolysis is carried out, the enzymatic hydrolysis solution is heated in a water bath, centrifuged, the supernatant is taken, and the polypeptide is obtained after freeze-drying.

This method makes use of the characteristics that guanidine thiocyanate can destroy the cell structure and denature the protein. It can not only extract nucleotides and peptides successively, but also has the advantages of simple method and high efficiency. At the same time, it also avoids the problem of reaction between biochemical reagents when adding biochemical reagents for extraction.