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What is the difference between urea, guanidine hydrochloride and guanidine isothiocyanate?
2022-05-13
The difference between urea, guanidine hydrochloride and guanidine isothiocyanate:
Concentration: At room temperature, 4~6mol/L urea and 3~4mol/L guanidine hydrochloride can make the globular protein change from the natural state to the midpoint of the denatured state. Usually, increasing the concentration of the denaturant can improve the degree of denaturation and denaturation, generally 8mol/ L urea and 6mol/L guanidine hydrochloride can completely convert the protein into a denatured state. However, some globular proteins could not be completely denatured even in 8 mol/L urea and 6 mol/L guanidine hydrochloride solutions, but continued to increase the solution concentration. There are conformational states.
Solubility: The solubility of urea is slower and weaker than that of guanidine hydrochloride, and the solubility of guanidine hydrochloride is slower and weaker than that of guanidine isothiocyanate. The solubility of urea is 70%~90%, and it will be cleaved to form cyanate if the action time is long or the temperature is high, and the amino group of the recombinant protein is covalently modified; price modification.
Concentration: At room temperature, 4~6mol/L urea and 3~4mol/L guanidine hydrochloride can make the globular protein change from the natural state to the midpoint of the denatured state. Usually, increasing the concentration of the denaturant can improve the degree of denaturation and denaturation, generally 8mol/ L urea and 6mol/L guanidine hydrochloride can completely convert the protein into a denatured state. However, some globular proteins could not be completely denatured even in 8 mol/L urea and 6 mol/L guanidine hydrochloride solutions, but continued to increase the solution concentration. There are conformational states.
Solubility: The solubility of urea is slower and weaker than that of guanidine hydrochloride, and the solubility of guanidine hydrochloride is slower and weaker than that of guanidine isothiocyanate. The solubility of urea is 70%~90%, and it will be cleaved to form cyanate if the action time is long or the temperature is high, and the amino group of the recombinant protein is covalently modified; price modification.
