The first mechanism is that the denatured protein binds preferentially to myochloride and urea to form a complex, when the complex is removed, the reaction equilibrium shifts, and as the concentration of the denaturant increases, the protein in its native state is continuously transformed into a com......
Read MoreConcentration: At room temperature, 4~6mol/L urea and 3~4mol/L guanidine hydrochloride can make the globular protein change from the natural state to the midpoint of the denatured state. Usually, increasing the concentration of the denaturant can improve the degree of denaturation and denaturation, ......
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